Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil
Article Abstract:
The method detection limits (MDL) of polymerase chain reaction (PCR) and immunofluorescence assay (IFA) for Cryptosporidium parvum oocysts in soil were investigated. Experiments involved soil characterization and seeding, recovery of oocysts from soils, DNA extraction and purification and PCR amplification. Results showed that the recovery efficiency for IFA is stable across orders of magnitude of seeding levels. In contrast, PCR is not effective as a screening step for soil studies of oocyst transport.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Hydrophobic and electrostatic cell surface properties of Cryptosporidium parvum
Article Abstract:
The low adhesion of oocysts of the parasite, Cryptosporidium parvum, to octane indicates that Cryptosporidium sp. have low hydrophobic properties. Analysis of hydrophobicity and zeta values indicates that pH and ionic strength affect the hydrophobicity. The maximum adhesion of the oocysts to octane is only 40%. The zeta potentials are pH-dependent and become less negative as pH drops.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Characterization of Cryptosporidium parvum by mastrix-assisted laser desorption ionization-time of flight mass spectrometry
Article Abstract:
Researchers report the first known use of MALDI-TOF mass spectroscopy to analyze protozoal oocysts. They found reproducible patterns of spectral markers that could distinguish Cryptosporidium parvum from other Cryptosporidium species.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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- Abstracts: A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk. Quantitative detection of Clostridium tyrobutyricum in milk by real-time PCR
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