Microbiological and molecular impacts of AbiK on the lytic cycle of Lactococcus lactis phages of the 936 and P335 species
Article Abstract:
Research has been conducted on the lactococcal abortive infection mechanism AbiK. Results indicate different phage responses towards the Abi system.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
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Phenotypic and genetic characterization of the bacteriophage abortive infection mechanism AbiK from Lactococcus lactis
Article Abstract:
The 10th abortive infection mechanism from Lactococcus lactis was isolated and characterized. Deletion mutants revealed that only orf1 was required to produce the Abi phenotype. orf1 (renamed abiK) coded for a predicted protein of 599 amino acids (AbiK) with an estimated molecular size of 71.4 kDa and a pI of 7.98. DNA and protein sequence alignment programs revealed no significant homology with databases. However, a database query based on amino acid composition indicated that AbiK may be in the same protein family as AbiA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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AbiQ, an abortive infection mechanism from Lactococcus lactis
Article Abstract:
A study was conducted to characterize a novel abortive-infection protein (Abi) from Lactococcus lactis and to analyze the impact of the Abi on the lytic cycle of the phage groups. Escherichia coli was determined at 37 degrees C in Luria broth while L. lactis was grown at 30 degrees C in an M17 solution supporting 0.5% glucose. Conventional analyses on DNA and putative proteins from the nucleic acid sequence were carried out using the Genetics Computer Group sequence analysis computer software.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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