Molecular biology of aflatoxin biosynthesis
Article Abstract:
Aflatoxins are biologically active secondary metabolites made by Aspergillus parasiticus, A. nomius and A. flavus fungi and can be deadly to humans if they are ingested. Genes involved in aflatoxin biosynthesis can be cloned to help generate understanding of the molecular biology that creates the toxins, as the cloned genes extract the molecular mechanisms that controls the expression and the timing of these genes. Analysis of the biosynthesis can provide insight on how to prevent the toxins from forming in foods.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Unsaturated fatty acids are the active molecules of a glucan-synthase-inhibitory fraction isolated from entomophthoralean protoplasts
Article Abstract:
The 1,3-beta-glucan synthase (GS) is inhibited by the unsaturated long chain fatty acids (FAs) existing in the extract from entomophthoralean, Entomophthora schizophorae, protoplasts. Oleic acid is the vital FA responsible for the inhibition. The inhibitory pathway is via the disruption of the membrane involving large amount of FAs which are non-specific and non-competitive. In the inhibitory fractions the fatty acids are separated by the thin-layer and gas chromatography.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Intracellular chitinase gene from Rhizopus oligosporous: Molecular cloning and characterization
Article Abstract:
Multiple chitinases have been observed in hyphae of filamentous fungi which are thought to have various hyphal growth function. The primary structure of chitinase III from Rhizopus oligosporous, a fungus, is reported. The deduced amino acid sequence of chi3 was similar to bacterial chitinases from mycoparasitic fungi. Chi3 was found to be transcribed during hyphal growth, suggesting a function during morphogenesis.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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