Molecular characterization of the genes pcaG and pcaH, encoding protocatechuate 3,4-dioxygenase, which are essential for vanillin catabolism in Pseudomonas sp. strain HR199
Article Abstract:
The molecular characteristics of the genes pcaG and pcaH of Pseudomonas sp. strain HR199 have been studied. Both genes encode protocatechuate 3,4-dioxygenase which is needed in the catabolism of vanillin. Nitrosoguanidine mutagenesis was used in producing mutants of the strain with disrupted vanillin catabolism but retain the ability to utilize eugenol or protocatechuate. Results revealed that vanillin is degraded through the ortho-cleavage pathway in Pseudomonas sp. strain HR199 while protocatechuate may be metabolized via different pathways in the mutants.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Highly efficient biotransformation of eugenol to ferulic acid and further conversion to vanillin in recombinant stains of Escherichia coli
Article Abstract:
The genes ehyAB, calA, and calB of Pseudomonas sp. strain HR199 were expressed in Escherichia coli to establish eugenol biotransformation to ferulic acid in the strain. By using the gene vaoA, a highly efficient biotransformation of eugenol to ferulic acid was established with recombinant strains of Escherichia coli on a technical scale.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Biochemical and genetic analyses of ferulic acid catabolism in Pseudomonas sp. strain HR199
Article Abstract:
Research conducted on the involvement of the fcs, ech and aat genes in ferulic acid conversion into vanillin in Pseudomonas sp. strain HR199 is analyzed. Experiments reveal gene disruption and mutant characterization during ferulic acid and eugenol catabolism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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