Molecular characterization of the levansucrase gene from the endophytic sugarcane bacterium Acetobacter diazotrophicus SRT4

Article Abstract:

The levansucrase (lsdA) gene of the gram-negative nitrogen-fixing bacteria, Acetobacter diazotrophicus SRT4, contains ribosome-binding regions, two putative translation initiation codons, and a transcription terminator. Its amino acid sequence specially in the C-terminal region is similar to that of the levansucrases of other Gram-negative bacteria. The gene encodes a 64.9 kDa polypeptide with levansucrase activity. Levansucrase is involved in sucrose metabolism, as a mutation in the lsdA gene disrupts levansucrase activity and the ability to use sucrose as a carbon source.

Observations, Gram-negative bacteria, Amino acid sequence, Amino acid sequencing

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Characterization of the rate-limiting step of the secretion of Bacillus subtilis alpha-amylase overproduced during the exponential phase of growth

Article Abstract:

A research on the overproduced secretion of Bacillus subtilis alpha-amylase in a degU32(Hy) genetic context was conducted. B. subtilis was subjected to a bacteria strain process, which was found to be homologous with the process administered with levansucrase. The findings of the study provided that sucrose induced the overproduction of lambda-Amylase by strain GM96101. It was also found out that any precursor from of lambda-amylase was not detected. Furthermore, the permeability of amylase on the cell wall depends on parietal properties.

Growth, Bacteria, Protein synthesis, Amylases

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Bacillus subtilis levansucrase: the efficiency of the second stage of secretion is modulated by external effectors assisting folding

Article Abstract:

The second stage of secretion of Bacillus subtilis levansucrase was prompted by the appearance of the H+ ion or Ca2+ ions in culture mediums of different pH levels, and was more efficient in more acidic levels. More basic pH levels required the presence of Ca2+ ions to sufficiently secrete the extracellular enzyme from the bacterium. Other proteins may be secreted from the bacterium, based on the results of the effects that H+ and Ca2+ ions presented.

Cell membranes, Protein folding

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