Molecular cloning, nucleotide sequence, and expression in Escherichia coli of a hemolytic toxin (aerolysin) gene from Aeromonas trota
Article Abstract:
Molecular cloning and the complete nucleotide sequence of the hemolytic toxin (aerolysin) gene, aerA, of Aeromonas trota and its expression in Escherichia coli are investigated. A conjugative suicide delivery plasmid was used to mutagenize A. trota AK2 to produce another mutant strain. Different aerolysin expression was obtained from both clones which reflect the role of intact and transposon-adjacent structural gene to the hemolysin expression. Moreover, it was also observed that E. coli cells containing various mutants were unable to produce any extracellular hemolytic activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Cloning, nucleotide sequence, and expression in Escherichia coli of levansucrase genes from the plant pathogens Pseudomonas syringae pv. glycinea and P. syringae pv. phaseolicola
Article Abstract:
The cloning and nucleotide sequencing of the Isc genes from Pseudomonas syringae pv. glycinea PG4180 and P. syringae pv. phaseolicola NCPPB 1321 are described. Levansucrase activity in Escherichia coli harboring Isc was detected mostly in the periplasmic fractions and to some extent in the membrane and cytoplasmic fractions but hardly in the extracellular fraction. Results indicate that Isc genes from gram-negative plant pathogens form a distinct cluster.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Biochemical and molecular characterization of tetracycline-resistant Aeromonas veronii isolates from catfish
Article Abstract:
Tetracycline resistant Aeromonas sp. strains (81 strains) were isolated from farm-raised catfish and its biochemical and molecular characteristics were studied. Results indicate the need for use of 16S rRNA in the identification of Aeromonas ssp. and the prevalence of catfish as a reservoir of tet genes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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- Abstracts: Molecular cloning, sequencing, and expression of a chemoreceptor gene from Leptospirillum ferrooxidans. Copper ions stimulate polyphosphate degradation and phosphate efflux in Acidithiobacillus ferrooxidans
- Abstracts: Cloning and analysis of the restriction-modification system LlaBI, a bacteriophage resistance system from Lactococcus lactis subsp. cremoris W56