Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence
Article Abstract:
Sequencing of the Clostridium botulinum type B neurotoxin gene revealed that the neurotoxin was composed of 1,291 amino acids. The L chain was most homologous to that of tetanus toxin and the H chain to that of the type A neurotoxin. A high degree of amino acid conservation is present, most notably that of tryptophan in the H chain. The most divergent region is the extreme carboxy terminus for each toxin, reflecting differing specificities for neuronal acceptor sites.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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A gene system for glucitol transport and metabolism in Clostridium beijerinckii NCIMB 8052
Article Abstract:
Clostridium beijerinckii possesses a gene system that codes for proteins necessary for glucitol transport and metabolism. These genes, designated as gut, were cloned on a chromosomal fragment from Escherichia coli and were found to occupy a region measuring four kilobases. There are five open reading frames in the cloned DNA fragment. The regulation of the expression of these genes is discussed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Analysis of the mechanism and regulation of lactose transport and metabolism in Clostridium acetobutylicum ATCC 824
Article Abstract:
The uptake and metabolism of lactose, the major sugar in industrial whey waste, by Clostridium acetobutylicum ATCC 824 is investigated. Lactose is taken up via a phosphoenolpyruvate-dependent phosphotransferase system and a pathway is proposed for the uptake and metabolism of lactose by an industrially important organism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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- Abstracts: Epitope regions in the heavy chain of Clostridium botulinum type E neurotoxin recognized by monoclonal antibodies
- Abstracts: Alternative reading frames of the INK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest
- Abstracts: Transcription of the glnB and glnA genes in the photosynthetic bacterium Rhodospirillum rubrum. Characterization of the glnB gene product of Nostoc punctiforme strain ATCC 29133:glnB or the P(sub II) protein may be essential
- Abstracts: Splicing defects in the ataxia-telangiectasia gene, ATM: underlying mutations and consequences. Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome
- Abstracts: Engineering of plasmin-resistant forms of streptokinase and their production in Bacillus subtilis: streptokinase with longer functional half-life