Monitoring impact of a pesticide treatment on bacterial soil communities by metabolic and genetic fingerprinting in addition to conventional testing procedures
Article Abstract:
Research was conducted to examine the impacts of a pesticide treatment on bacterial soil communities taking herbogil, a reference herbicide, the mineral oil Oleo and Goltix as model compounds. The effects on metabolic sum parameters were established through the monitoring of substrate-induced respiration and dehydrogenase activity and nitrogen and carbon mineralization. The BIOLOG system and pattern generation by amplified ribosomal sequences were used to analyze the suitability of the approaches for the analysis of the impacts of herbicide applications.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium
Article Abstract:
A new hybridization-based screening method for bacterial 16S rDNA clone libraries is used in studying an anaerobic, trichlorobenzene-transforming microbial community. Small-subunit rRNA genes were amplified using polymerase chain reaction from community DNA by using primers specific for Bacteria or Euryarchaeota and the genes were subsequently cloned. The new screening approached detected 51 bacterial clone families and one clone family was closely related to the dechlorinating Dehalobacter species.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Peptide nucleic acid-mediated PCR clamping as a useful supplement in the determination of microbial diversity
Article Abstract:
Results demonstrate that polymerase chain reaction clamping technique can be employed in the analysis of microbial diversity in complex microbial communities. Data indicate that the technique functions as a supplement to the statandard polymerase chain reaction amplification in ribosomal-DNA based studies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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- Abstracts: Biodegradation of free phytol by bacterial communities isolated from marine sediments under aerobic and denitrifying conditions
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- Abstracts: Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure. Enhancement of secretion and extracellular stability of staphylokinase in Bacillus subtilis by wprA gene disruption
- Abstracts: Cloning, expression, and characterization of aminopeptidase P from the hyperthermophilic archaeon Thermococcus sp. strain NA1
- Abstracts: Formation of bound residues during microbial degradation of (14C)anthracene in soil. Naphtalene degradation and incorporation of naphthalene-derived carbon into biomass by the thermopile Bacillus thermoleovorans