Mosquitocidal activity of the CryIC delta-endotoxin from Bacillus thuringiensis subsp. aizawai
Article Abstract:
The CryIC delta-endotoxin produced by Bacillus thuringiensis subsp. aizawai is a dually-active toxic as it kills mosquitoes of the Lepidoptera and Diptera species. The endotoxin is toxic for larvae of three mosquito species unlike the CryIA(a) and CryIA(c) endotoxins. Soluble, proteolytically stimulated CryIC delta-endotoxin is also toxic for mosquito derived cell lines. Truncation of the C-terminal of the endotoxin makes it nontoxic as it becomes insoluble in alkaline reducing conditions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Role of proteolysis in determining potency of Bacillus thuringiensis Cry1Ac delta-endotoxin
Article Abstract:
Some insects may be resistant to the Cry1Ac delta-endotoxin of Bacillus thuringiensis because they contain intestinal proteases that completely degrade the toxin. Some degree of proteolysis is necessary to activate the toxin inside the insect, but too much can inactivate the toxin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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The alpha-helix 4 residue, Asn135, is involved in the oligomerization of Cry1Ac1 and Cry1Ab5 Bacillus thuringiensis toxins
Article Abstract:
Residue Asn135 in alpha-helix 4 of the Cry1Ac1 and Cry1Ab5 Bacillus thuringiensis toxins is necessary for pore formation and toxin oligomerization in the gut epithelium of Manduca sexta. However, a mutant protein created by substituting Gln for Asp still binds to the epithelium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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