Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1
Article Abstract:
A new reporter gene fusion, ACT1-CUP1, to monitor pre-mRNA splicing through copper-resistant growth assays was developed. In this study, the S. cerevisiae metallothionein homolog CUP1 was cloned from an intron-containing fragment from the S. cerevisieae ACT1 gene. An assay for 5' splice sites was developed and mutations were introduced to test the functions of the highly conserved nucleotides in intron recognition. Results showed that most mutations in the intron part of the 5' sites abolished recognition of the mutated site.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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Isolation and characterization of SGE1: a yeast Gene that partially suppressesthe gal11 mutation in multiple copies
Article Abstract:
Researchers isolated a gene, SGE1, that suppresses GAL11 for development on ethidium bromide/galactose agar at the time the gene exists in two or more copies. Recessive mutation of GAL11 in Saccharomyces cerevisiae results in pleiotropic defects that involve weak fermentation of galactose, alpha specific sterility and slow development on nonfermentable carbon sources. GAL11 P operates as a coactivator that connects transcriptional activators such as Gal4p and Grf1p/Rap1p/Tuf1p with fundamental transcription equipment.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
User Contributions:
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