Natural genetic transformation: A novel tool for efficient genetic engineering of the dairy bacterium Streptococcus thermophilus
Article Abstract:
A system based on natural transformation was developed to make genetic manipulations in Streptococcus thermophilus easy, rapid and highly efficient. It was established that the efficiency of the novel tool would make it possible to construct food-grade mutants of Streptococcus thermophilus, thus opening up exciting new possibilities to benefit consumers as well as dairy industry.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Antisense RNA targeting of primase interferes with bacteriophage replication in Streptococcus thermophilus
Article Abstract:
The effects of primase-targeted antisense RNA on phage development are examined. Regions responsible for antisense efficacy were determined through the characterization of a variety of constructs engineered to constitutively express antisense RNA complementary to various structural or putative regulatory regions of pri3.1.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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thyA as a selection marker in construction of food-grade host-vector and integration systems for Streptococcus thermophilus
Article Abstract:
Streptococcus thermophilus is one of the important lactic acid bacteria (LAB) and has long been used to produce yogurt and cheeses. A description of how spontaneous thymidylate synthase gene(thyA) mutants of Streptococcus thermophilus are obtained by using high concentrations of trimethoprim(TMP) is provided.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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