Nested PCR for ultrasensitive detection of the potato ring rot bacterium, Clavibacter michiganensis subsp. sependonicus

Article Abstract:

Nested polymerase chain reaction (PCR) was used for the ultrasensitive detection of the potato ring rot bacterium, Clavibacter michiganensis subsp. sependonicus and the development of PCR assays with primers based on the highly repeated segment of DNA. Results show that PCR assays for the detection of C. michiganensis subsp. sependonicus in commercial potato tubers were consistent and accompanied by the presence of low titer of bacterium. Furthermore, there is a difference in the efficiency in the bacterium depending on the primers used in the nested PCR.

Bacteria, Polymerase chain reaction, Ribosomal RNA

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Stable transformation of the gram-positive phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus with several cloning vectors

Article Abstract:

Cloning vectors are developed by using several plasmids in the transformation of Clavibacter michiganensis subsp. sepedonicus, the potato ring rot bacterium. Results show that different strains of C. michiganensis subsp. sepedonicus exhibit different transformation efficiencies for a single plasmid. The data also suggest that optimum transformation efficiency can be achieved by growing C. michiganensis subsp. sepedonicus cells on DM agar plates harvested during the early exponential growth phase.

Analysis, Cloning, Pathogenic microorganisms, Bacterial transformation

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Purification, characterization, and gene sequence of Michiganin A, an actagardine-like lantibiotic produced by the tomato pathogen Clavibacter michiganensis subsp. michiganensis

Article Abstract:

The antimicrobial peptide michiganin A was isolated from the tomato pathogen 'Clavibacter michiganensis' subsp. 'michiganensis' for purposes of purification and characterization. Michiganin A was seen to be a type B lantibiotic and cloning and sequencing of the gene additionally revealed the sequence of the atypical prepeptide of michiganin A.

Science & research, Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Antiinfective Preparations, Norway, Physiological aspects, Anti-infective agents, Genetic aspects, Bacteria, Pathogenic, Pathogenic bacteria, Genetic research, Bacteriocins

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