Flow cytometric analysis of activated sludge with rRNA-targeted probes
Article Abstract:
Using a combination of a flow cytometry (FCM) and rRNA-targeted probes the number of bacterial cells present in activated sludge and their metabolic activities was determined fast and accurately. FCM determines the physical and chemical properties of the cells on the basis of light scattering and fluorescence produced by the dyes bound to the bacterial cells. Using this method 70% to 80% of the cells could be identified, most of which belonged to the beta-subclass Proteobacteria. However, the bacteria present in flocs or chains could not be accurately identified.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp.
Article Abstract:
The primary sequence of the 16S and 23S rRNA of the obligate intracellular bacterial parasites of acanthamoebae is 86 to 87% similar to that of the genus Chlamydia. The parasites probably constitute another genus of Chlamydiaceae rather than another species. The rRNA analysis and in situ hybridization with a horseradish peroxidase-labeled oligonucleotide identifies a site that assigns the 16S rRNA sequence to the respective intracellular parasite. The results of the study show that small amoebae act as environmental reservoirs and vectors for pathogenic bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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High bacterial diversity in permanently cold marine sediments
Article Abstract:
Research was conducted to examine the phylogenetic affiliation and diversity of the prokaryotic community and to quantify the contribution of sulfate-reducing bacteria to total microbial community. A sample of 30 clones was chosen for sequencing and phylogenetic analysis to investigate diversity in the 16S rDNA clone library. Results indicate that the total diversity evaluated by the 16S rDNA method was very large in the permanently cold sediments and was only partially observed through the screening of 3553 clones.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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