Organization around the dnaA gene of Streptococcus pneumoniae

Article Abstract:

The dnaA gene region of Streptococcus pneumoniae was cloned and sequenced and a tRNA gene, seven open reading frames (ORFs) and three DnaA box clusters were determined. The sequence of the genes and intergene regions was tRNA(super Arg)-orf1-DnaA box cluster 3-htrA-spoOJ-DnaA box cluster 2-dnaA-DnaA box cluster 1-dnaN-orfX-orfY. The N-terminal portion of orfY had high homology with a GTP-binding protein. DnaA box clusters were observed upstream and downstream from dnaA. The presence of two such clusters indicated that the chromosomal origin of S. pneumoniae is found within this region.

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Exclusion of long heterologous insertions and deletions from the pairing synapsis in pneumococcal transformation

Article Abstract:

The mode of recombination of six heterologous insertions during the genetic transformation of Streptococcus pneumoniae is investigated. The insertions are of different sizes and are done on the same site in the pneumococcal chromosome. Results indicate a correlation between the magnitude of the shift and the size of the insert. Further experiments reveal that long heterologous insertions are partly excluded at the pairing step.

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Electrotransformation and natural transformation of Streptococus pneumoniae: requirement of DNA processing for recombination

Article Abstract:

Research was conducted to examine the recombination of markers in the chromosome of the naturally transformable bacterium Streptococcus pneumoniae using the electrotransformation technique. Naturally competent cultures, chromosomal and cloned DNA were used but did not lead to recombination. Results indicated that to generate recombinants in S pneumonia, it is not sufficient just to introduce DNA into the cell.

DNA, Plasmids

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