Phenol- and toluene-degrading microbial populations from an aquifer in which successful trichloroethene cometabolism occurred
Article Abstract:
The bacterial populations that grew in a Moffett Field, CA, aquifer were characterized after three sequential field tests of phenol- or toluene-driven cometabolism of trichloroethene (TCE). TCE was cometabolized by 60% of the genetically-different strains. Most strains grew on both phenol and toluene, and 78% of these strains hybridized to the toluene oxygenase pathways. Gram-positive strains constituted 30% of the collection. A larger percentage of TCE degraders were observed among gram-negative than gram-positive strains and among organisms that grew on phenol than those that grew on toluene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Microbial succession during a field evaluation of phenol and toluene as the primary substrates for trichloroethene cometabolism
Article Abstract:
The composition and succession of a microbial community were investigated in an aquifer in which phenol, toluene and chlorinated aliphatic hydrocarbons were added to assess the effectiveness of these aromatic substrates to stimulate trichloroethene bioremediation. Findings revealed that six of the seven originally dominant microbial groups remained dominant after the last treatment, suggesting that the community is quite immune to toxic disturbance by 1,1-dichloroethene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Characterization of the structural gene encoding a copper-containing nitritereductase and homology of this gene to DNA of other denitrifiers
Article Abstract:
The primary structureand the DNA-gene homology of a copper-containing nitrite reductase structural gene is discussed. This gene was derived from the Pseudomonas sp. strain and was subjected to genomic DNA isolation, restriction enzyme digestion, electrophoresis and Southern blotting. The utility of the structural gene as a probe for detecting denitrifiers with copper-containing nitrite reductases was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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