Assessment of microbial diversity in four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis
Article Abstract:
Research has been conducted on the complex microbial communities. The relationship between the microbial communities in environmental samples has been investigated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
Phylogenetic specificity and reproducibility and new method for analysis of terminal restriction fragment profiles of 16S rRNA genes from bacterial communities
Article Abstract:
An analytical procedure is presented for improving outcomes from replicate terminal restriction fragment profiles used in DNA testing of microbial communities. The procedure involves sample size standardization, which, although it has many practical applications, may be inappropriate for samples that are disparately low in DNA quantities compared to other samples in the set.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Levels of bacterial community diversity in four arid soils compared by cultivation and 16S rRNA gene cloning
Article Abstract:
Levels of bacterial community diversity in two pinyon rhizosphere soil samples and two between-tree soil samples have been compared using methods based on amplification of 16S rRNA genes. To this end, 179 cultivated bacterial isolates and 801 16S rRNA genes amplified from extracted soil DNA were analyzed. Results show a total of 34 phylotypes identified among the cultivated isolates while 498 phylotypes occurred among the 16S ribosomal DNA clones. The levels of phylotype similarity between bacterial communities were uniformly low.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil
- Abstracts: Novel tellurite-amended media and specific chromosomal and Ti plasmid probes for direct analysis of soil populations of Agrobacterium biovars 1 and 2
- Abstracts: Mutation of the ptsG gene results in increased production of succinate in fermentation of glucose by Escherichia coli
- Abstracts: Characterization of emetic Bacillus weihenstephanensis, a new cereulide-producing bacterium. Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis