Potential of conjugal transfer as a strategy for the introduction of recombinant genetic material into strains of Lactobacillus helveticus
Article Abstract:
The ease with which a pIP501-derived recombinant plasmid cointegrate could be transferred to a range of strains of Lactobacillus helveticus was investigated and the expression and stability of the cloned gene in different strains was assessed. The plasmid cointegrate formed seemed to be segregationally stable at 37 degrees C in some host strains, but in all strains became increasingly unstable as the incubation temperature was increased. The method represents a generalized approach for introducing novel genetic material into L helveticus and offers the possibility of exploiting the thermal sensitivity of the plasmid to permit it to act as a delivery system for the integration of cloned genes into the bacterial chromosome by recombination at regions of homology.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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High-frequency plasmid transduction by Lactobacillus gasseri bacteriophage phi(adh)
Article Abstract:
The BglII fragments of the temperate phage phi(adh) DNA were cloned into the BclI site of the transducible plasmid vector pGK12 and the influence of phi(adh) on plasmid transduction was determined. The study showed that the inserted phi(adh) fragments induced the formation of high-molecular weight forms of recombinant pGK12 plasmids. The extent of DNA-DNA homology between these plasmids and phage phi(adh) DNAs were then found to be correlated with the increase in transduction frequencies owing to the formation of high-frequency transducing phage particles.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Expression, regulation, and mode of action of the AbiG abortive infection system of Lactococcus lactis subsp. cremoris UC653
Article Abstract:
The expression and regulation of the genes abiGi and abiGii found in the lactococcal AbiG system have been studied. AbiG is an abortive infection system encoded by the lactococcal plasmid pCI750. Northern blot analysis found two transcripts of approximately 2.8 and 1.5 kb. The two genes are co-transcribed resulting in a 2.8-kb transcript. The smaller transcript may have resulted from the independent transcription of abiGii within abiGi or of the presence of a weak terminator within abiGii.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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