Production of thermostable direct hemolysin by Vibrio parahaemolyticus enhanced by conjugated bile acids

Article Abstract:

Bile acids promote synthesis and secretion of thermostable direct hemolysin (TDH) in Vibrio parahaemolyticus. TDH secretion in V. parahaemolyticus, cultured in the presence of bile components taurocholic acid (TC) and glycocholic acid (GC), increases significantly. TDH in the lysates of cells grown in the presence of TC and GC is also significantly higher than the control, indicating that the bile acids enhance the synthesis of TDH within the bacterial cells. Thus, conjugated bile acids play a significant role in the pathogenicity of V. parahaemolyticus in the human intestine.

Physiological aspects, Bile acids

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Soft-agar-coated filter method for early detection of viable and thermostable direct hemolysin (TDH)- or TDH-related hemolysin-producing Vibrio parahaemolyticus in seafood

Article Abstract:

A novel method was developed to detect viable and thermostable direct hemolysin (TDH) producing or TDH-related hemolysin (TRH)-producing Vibrio parahaemolyticus in seafood. The method employs two principals, one being that only viable Vibrio parahaemolyticus cells could penetrate through a soft-agar-coated filter because of their motility and the other being that DNAs released from ruptured virulent Vibrio parahaemolyticus cells into enrichment medium can be avoided by DNase pretreatment so that they do not interfere with subsequent multiplex PCR.

Science & research, Methods, Food, Genetic aspects, Food testing

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Occurrence of urease-positive Vibrio parahaemolyticus in Kanagawa, Japan, with specific reference to the presence of thermostable direct hemolysin (TDH) and the TDH-related-hemolysin genes

Article Abstract:

All the urease positive (UH+) strains of Vibrio parahaemolyticus isolated from patients and food samples from Kanagawa, Japan, possess the thermostable direct hemolysin (tdh) -related-hemolysin gene. UH+ activity is not a reliable indicator of the presence of tdh gene as only 6% of the strains possessing the tdh gene are UH+. The 132 tested strains belong to eight of the twelve known serogroups and O4 is the major serogroup. UH+ activity is present in ten strains of five serotypes.

Identification and classification

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