Protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from Burkholderia sp. strain DNT for enhanced degradation of nitroaromatics

Article Abstract:

4-Methyl-5-nitrocatechol (4M5NC) monooxygenase (DntB) from Burkholderia sp. strain DNT turns the second step of 2,4-dinitrotoluene degradation by converting 4M5NC to 2-hydroxy-5-methylquinone with the concomitant removal of the nitro group. DntB is a flavoprotein that has a very narrow substrate range and error-prone PCR is applied to create variant DntB M22L/L380I that accepts two new substrates 4-nitrophenol (4NP) and 3-methyl-4-nitrophenol (3M4NP).

Cyclic Crude and Intermediate Manufacturing, Nitrated Aromatics, Analysis, Bacterial genetics, Decomposition (Chemistry), Nitroaromatic compounds

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An inducible propane monooxygenase is responsible for N-nitrosodimethylamine degradation by Rhodococcus sp. strain RHA1

Article Abstract:

The effects of the propane on the gene expression and biological removal of the water contaminant N-nitrosodimethylamine (NDMA) are examined. The results have shown that propane monooxygenase is responsible for the degradation of NDMA by Rhodococcus sp. strain RHA1 and have explained the increased cometabolic degradation of NDMA in the presence of propane.

Gene expression, Propane, Nitrosoamines

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Protein engineering of toluene-o-xylene monooxygenase from Pseudomonas stutzeri OX1 for synthesizing 4-methylresorcinol, methylhydroquinone and pyrogallol

Article Abstract:

Dihydroxy and trihydroxy aromatic compounds, are industrial chemicals which cannot be easily combined chemically and the use of old methods are expensive. An experiment is conducted wherein these compounds are combined with benzene, toluene etc to produce valuable hydroxylated benzenes.

Protein engineering, Aromatic compounds

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