Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost

Article Abstract:

Research was conducted to purify and characterize three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost. The microorganism was observed to grow only in colloidal chitin supplemented with yeast extract and (2,6-O-dimethyl)-beta-cyclodextrin and not on any of the usual media for actinomyces. Results indicate that the three enzymes were inert to pNP-GlcNAc and that their activities were inhibited by AgCl, (GlcNAc)2 and HgCl2.

Bacterial growth, Bacillus (Bacteria), Bacillus, Compost, Bacteriology, Bacterial cultures

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Specific cell wall proteins confer resistance to nisin upon yeast cells

Article Abstract:

A study was conducted to analyze the importance of individual mannoproteins and chitin in conferring resistance to nisin upon yeast cells. Yeast cells were obtained by centrifugation and grown in a YPD medium at 25 degrees C. RNA was then isolated using the hot phenol extraction technique. Results indicated that cell way protein 2 supports a critical function in protecting cells against antimicrobial peptides.

Analysis, Cells (Biology), Peptides, Proteins, Yeast, Yeast (Food product), Cells

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Antimicrobial actions of degraded and native chitosan against spoilage organisms in laboratory media and foods

Article Abstract:

Chitosan has some antimicrobial activity in some foods, but it may not have wide applicability as a food preservative. Partial hydrolysis increases its antimicrobial activity slightly but at the expense of extra processing.

Food, Protection and preservation, Food preservation

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