Purification and properties of an ATP-dependent nucleosome remodeling factor
Article Abstract:
The ATP-dependent nucleosome remodeling factor (NURF) from Drosophila embryo consists of four polypeptides that interact with the GAGA transcription factor to change the chromatin structure at the hsp 70 promoter. The effect of NURF on nucleosome arrays is more than that on individual nucleosomes. NURF acts directly on the nucleosomes as the energy required for the ATPase activity is obtained from the nucleosomes and not free DNA or histones. The substrate recognition and stimulation mechanism of NURF is different from that of the SWI/SNF protein complex which also changes the chromatin structure.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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ISWI, a member of the SW12/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodelling factor
Article Abstract:
The Drosophila imitation switch (ISWI) protein is a subunit of an ATP dependent nucleosome remodelling factor (NURF) and is structurally similar to the ATPases of the SWI2/SNF2 family. ISWI is present in the nucleus and is expressed during development. The GAGA transcription factor and NURF are required for the generation of heat shock promoter in chromatin. Both, NURF and the SWI/SNF ATPases alter the chromatin structure but, their action mechanism are different.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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Characterization of the Imitation Switch subfamily of ATP-dependent chromatin-remodeling factors in Saccaromyces cerevisiae
Article Abstract:
ATP-dependent chromatin-remodeling activities are central to the in vivo functions of the Imitation Switch complexes identified as ISW1 and ISW2. Research of the Saccaromyces cerevisiae yeast complexes shows multiple subunit complexes, a single point mutation within which inactivates all ATP-dependent biochemical activities as well as the genes' ability to rescue mutant phenotypes.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1999
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