Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments
Article Abstract:
Viable bacterial cells can easily be counted by inducing spheroplast formation using antibiotics and glycine. The resulting swollen cells are lysed by a single freeze-thaw treatment. The cells that remain are subtracted from the total number before the procedure to give the number of viable cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Rapid and simple quantification of bacterial cells by using a microfluidic device
Article Abstract:
The study investigated a microfluidic chip-based system for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Quantitative determination of free-DNA uptake in river Bacteria at the single-cell level by in situ rolling-circle amplification
Article Abstract:
Rolling-circle amplification (RCA) was used to detect plasmid DNA uptake in river bacteria at the single-cell level. In situ RCA gave the ability to detect the uptake of plasma DNA with better accuracy than the direct counting cells expressing the gfp gene under fluorescence microscopy.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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