Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR
Article Abstract:
The viability of using polymerase chain reaction (PCR) in detecting sapstain fungi in wood samples has been investigated. Ophiostoma piceae is used as the model organism because it is the most common sapstain fungi species isolated from stained lumber and logs. However, the fungi species has been divided between O. piceae for hardwoods and O. quercus for softwoods. Detecting both fungal species was achieved within four hours through a combination of microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Biological degradation of resin acids in wood chips by wood-inhabiting fungi
Article Abstract:
Experimental studies on lodgepole pine sapwood chips, which are inoculated with four different fungal strains, indicate that fungal growth reduces up to 67% of the total resin acids level, which are primary sources of toxicity to fish. Use of wood-inhabiting fungi is an efficient and environmental friendly method for the biological degradation of resin acids in wood chips. This methodology facilitates the removal of toxicity of pulp mill effluents.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Disruption of the subtilase gene, albin1, in Ophiostoma piliferum
Article Abstract:
Three different groups are found in subtilases, which are located in the sapstaining fungi, but it is not clear whether these groups have specific functions to be performed. The results of the study undertaken to identify the function of subtilase albin1 from Ophiostoma piliferum shows that the albin1 has significant functions to be performed in Ophiostoma piliferum's capability to procure nitrogen from wood proteins.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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