Replication-dependent sister chromatid recombination in rad1 mutants of Saccharomyces cerevisae
Article Abstract:
RAD1 is needed for incision in the excision repair pathway required for removal of most UV damage in yeast. Sister chromatid and homolog recombination induced by UV treatment of RAD1/rad1 and rad1/rad1 strains of Saccharomyces cerevisiae cells at different times in the cell cycle were assessed. High levels of replication-dependent UV-induced sister chromatid recombination were found in rad1/rad1 mutants whether UV was administered in G1 or G2. Sister chromatid recombination after UV irradiation in G2, on the other hand, was observed in RAD1/rad1 cells with the G1-induced UV lesions probably removed prior to onset of replication.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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Sister chromatids are preferred over homologs as substrates for recombinational repair in Saccharomyces cerevisiae
Article Abstract:
Diploid Saccharomyces cerevisiae were synchronized in either the first (G1) or the second (G2) growth phase and irradiated with X ray to study homolog and sister chromatid recombinations. Yeast exposed to radiation at the G1 repaired X ray-induced damage preferentially by homolog recombination while G2-irradiated yeasts repaired DNA damage by sister chromatid recombination. Further, yeast irradiated at G1 were much more susceptible to lethal X ray-induced DNA damage. It was concluded that sister chromatids are preferred as substrates for recombination and can reconstruct more DNA damage than homologs.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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a/alpha-control of DNA repair in the yeast Saccharomyces cerevisiae: genetic and physiological aspects
Article Abstract:
The effect of the mating type (MAT) locus of Saccharomyces cerevisiae on DNA repair and the a/alpha-dependent component of diploid repair by means of the a1 and alpha2 alleles were studied. Results showed that MAT heterozygosity is needed for resistance of diploid cells to gamma rays. Both a and alpha affect DNA metabolism in G2 mitotic phase, independent of RME1. Further studies showed that a1-alpha2 promotes channeling of some DNA structures from mutagenic into recombinational repair.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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- Abstracts: MET17 and hydrogen sulfide formation in Saccharomyces cerevisiae. A novel gene encoding xanthan lyase of Paenibacillus alginolyticus strain XL-1
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