PhaG-mediated synthesis of poly(2-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi
Article Abstract:
PhaG-mediated synthesis of poly(2-hydroxyalkanoates) is discussed. This synthesis uses medium-chain-length parts of nonrelated carbon sources in recombinant Pseudomonas fragi. PHA(sub.MCL) synthesis has been established from nonrelated carbon sources in a non-PHA-accumulating bacterium, using fatty acid de novo biosynthesis and the enzymes PhaG and PhaC1
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Role of fatty acid de novo biosynthesis in polyhydroxyalkanoic acid (PHA) and rhamnolipid synthesis by pseudomonads: establishment of the transacylase (PhaG)-mediated pathway for PHA biosynthesis in Escherichia coli
Article Abstract:
The enzyme 3-hydroxydecanoyl-ACP-CoA transacylase links fatty acid de novo biosynthesis to polyhydroxyalkanoic acid synthesis in Escherichia coli. This pathway is competitive with the production of rhamnolipid from de novo fatty acid biosynthesis, which is catalyzed by beta-ketoacyl reductase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Engineering the genotype of Acinetobacter sp. strain ADP1 to enhance biosynthesis of cyanophycin
Article Abstract:
Genes encoding the putative arginine regulatory protein (argR) and the arginine succinyltransferase (aster) were inactivated and the effects of these mutations on CGP synthesis were analyzed, to study the importance of arginine provision and phosphate limitation for synthesis and accumulation of cyanophycin (CGP) in Acinetobacter sp. strain ADP1. The inactivation of the genes resulted in a 3.5- or 7-fold increase in CGP content respectively when the cells were grown on glutamate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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