Role of microbial immigration in the colonization of apple leaves by Aureobasidium pullulans
Article Abstract:
The green fluorescent protein (GFP)-tagged blastospores to the foliage of orchard trees at early and late seasons was applied to investigate the role of microbial immigration in the veinal colonization pattern of Aureobasidium pullulans on the adaxial surface of apple leaves. The results indicate that growth-promoting substances, which occur locally in the veinal areas, are indirectly responsible for Aureobasidium pullulans veinal colonization patterns in the field and not the factors associated with immigration limitation and growth-inhibition.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Development of an oligonucleotide probe for Aureobasidium pullulans based on the small-subunit rRNA gene
Article Abstract:
The nucleotide sequences of the small-subunit rRNA genes of 12 geographically diverse Aureobasidium pullulans strains are analyzed to evaluate the vulnerability of rRNA to A. pullulans-specific oligonucleotide probes. Results show that a short oligonucleotide with a single nucleotide mismatch to the target sequence can differentiate strains. This finding suggests that the oligonucleotide probes can be used in quantifying fungi in leaf surface and other microbial communities.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Temporal changes in microscale colonization of the phylloplane by Aureobasidium pullulans
Article Abstract:
Colonization of apple leaves by the yestike fungus Aureobasidium pullulans is followed quantitatively and spatially at a microscale level throughout two growing seasons to document the spatial pattern of Aureobasidium pullulans. The results suggest that Aureobasidium pullulans primarily colonizes veins, where populations appear to increase by growth in situ, which is established early in the growing season and persists.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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