ScCypB is a novel second cytosolic cyclophilin from Streptomyces chrysomallus which is phylogenetically distant from ScCypA
Article Abstract:
Screening of Streptomyces chrysomallus cosmid gene library using the probe for cyclophilin gene sccypA led to the isolation and identification of a new cyclophilin gene, sccypB. Functional characterization of sccyB indicated that it has the same enzymatic activity and substrate specificity as that of sccypA, that is, they are both peptidyl-prolyl cis-trans isomerases (PPIase). Although sccyB is also sensitive to cyclosporin A, it was found to be three times less sensitive than sccyA. Western blot analysis also confirmed that sccyB is 50- to 100-times less abundant in the cytosol of bacterial cells. Phylogenetic tree assessment indicated that the two genes are phylogenetically distant from each other.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Genetically modified bacterial strains and novel bacterial artificial chromosome shuttle vectors for constructing environmental libraries and detecting heterologous natural products in multiple expression hosts
Article Abstract:
A new method that utilizes Streptomyces lividans and Pseudomonas putida as additional hosts with different expression capabilities and, which can be used for transferring environmental libraries into all three expression hosts is described. The use of the new method suggests that the three expression hosts may differ in their abilities to express gene encoding of chemically diverse small molecules.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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