Segregation of storage protein mRNAs on the rough endoplasmic reticulum membranes of rice endosperm cells
Article Abstract:
The mechanism for the segregation of storage proteins in developing rice endosperm cells was investigated. The study protocol involved blot hybridization and high-resolution in situ hybridization to visualize the spatial distributions and densities of storage protein mRNAs. The results showed that the initial targeting process of storage proteins to specific protein bodies is accomplished by segregating mRNA transcripts either to the cisternal endoplasmic reticula (ER) or to the protein body ER, with prolamine protein localizing to the latter.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Membrane protein biogenesis: Regulated complexity at the endoplasmic reticulum
Article Abstract:
A protein has to be accurately localized in space and time to undertake its intended function in a cell. The secretory pathway is used by almost all proteins destined for extracellular or membrane-bound locations within the eukaryotic cell. Secretory and membrane proteins move across the endoplasmic reticulum (ER) membrane, a translocation that starts in the cytosol. Current understanding of the molecular factors directing the translocation of simple secretory proteins, reveals a deceptively simple picture.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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A second signal recognition event required for translocation into the endoplasmic reticulum
Article Abstract:
Two different signal identification events, one with a signal recognition particle (SRP) and the other with proteoliposomes carrying the Sec61p complex have been found. In cotranslational protein transport, both identification events are found to be needed for effective translocation of the protein into the lumen of the endoplasmic reticulum (ER). The need for SRP can in some experimental situations be avoided by diminishing of nascent polypeptide-associated complex.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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