Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR
Article Abstract:
A reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection of low numbers of viable Giardia sporidium parvum and Cryptosporidium parvum in large-volume water samples is introduced. It is more sensitive than an infrared microscopy procedure and particularly detects the human pathogen C. parvum. The assay was found to be able to detect low numbers of organisms, in the range of a single viable cyst and oocyst, when spiked into 100 microliter packed pellet volumes of concentrates from creek and river water samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR
Article Abstract:
Reverse transcription-PCR, based on mRNA detection from a heat shock protein (hsp) of the Cryptosporidium parvum, is capable of detecting the presence of a single viable oocyst in concentrated environmental water samples. The reverse transcription method uses an amplification of a 590-bp region of the C.parvum hsp70 mRNA to distinguish live oocysts from dead ones. This method is an effective, economical, versatile, simple and rapid technique for the screening of waterborne pathogens.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Identification of Mycobacterium ulcerans in the environment from regions in Southeast Australia in which it is endemic with sequence capture-PCR
Article Abstract:
Researchers used magnetic bead sequence capture-PCR to analyze water samples from sites close to two outbreaks of Mycobacterium ulcerans disease in southeast Australia. A golf course irrigation system and a small lake both tested positive for the organism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Prevalence and identity of Cryptosporidium spp. in pig slurry
- Abstracts: Effect of pasteurization on infectivity of Cryptosporidium parvum oocysts in water and milk. In vitro interactions of Asian freshwater clam (Corbicula fluminea) hemocytes and Cryptosporidium parvum oocysts
- Abstracts: Germination, growth, and sporulation of Bacillus thuringiensis subsp. israelensis in excreted food vacuoles of the protozoan Tetrahymena pyriformis
- Abstracts: Responses of methanotrophic activity in soils and cultures to water stress. Methanol promotes atmospheric methane oxidation by methanotrophic cultures and soils