Sequence and DNA structural determinants of N4 virion RNA polymerase-promoter recognition
Article Abstract:
N4, a bacteriophage, does not have to have host RNA polymerase (RNAP) to express its early genes. Coliphage N4-coded, virion-encapsidated RNA polymerase (vRNAP) can bind to and transcribe promoter-containing double-stranded DNA in cases where the template is supercoiled and in the presence of Escherichia coli single-stranded DNA-binding protein (Eco SSB). Hairpin structure on the template strand and certain sequences are necessary for vRNAP-promoter recognition and activity on the templates. A model for in vivo utilization of the vRNAP promoters has been suggested, as have certain possible roles for DNA supercoiling and Eco SSB in activating promoter.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
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E. coli SSB activates N4 virion RNA polymerase promoters by stabilizing a DNA hairpin required for promoter recognition
Article Abstract:
The bacterial virus N4 proliferates by locating short single-stranded loops on the host protein and initiating transcription in the presence of E.coli single-stranded DNA binding protein (EcoSSB). The specific requirements of the N4 RNA polymerase are due to its need to attach itself to a stable single-stranded hairpin loop. EcoSSB acts as the stabilizing element by binding to a promoter region in its hairpin form and renders it accessible to the viral polymerase.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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Transcripts that increase the processivity and elongation rate of RNA polymerase
Article Abstract:
The structure and function of the lambdoid phage HK022's antitermination sites are investigated. The sites have been named put for polymerase utilization. Data indicate that the active form of put is its RNA transcript. Evidence also indicates that the interaction of the transcript with a site on elongating RNA polymerase triggers suppression of termination and pausing.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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