Role of chitin-binding proteins in the specific attachment of the marine bacterium Vibrio harveyi to chitin
Article Abstract:
The mechanism for the specific attachment of marine bacterium Vibrio harveyi to chitin particles is discussed. Chitinase activity was measured as chitin and cellulose were treated with protein. Chitin-binding proteins and chitinase were isolated, electrophoretically separated and immunoblotted. It was found that chitin-binding peptides are used by V. harveyi to mediate attachment to chitin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Multiple genes involved in chitin degradation from the marine bacterium Pseudoalteromonas sp. strain S91
Article Abstract:
The cloning, sequencing and characterization of a cluster of three closely related chitinase genes organized in the order chiA, chiB and chiC having the same transcriptional direction, along with two unlinked genes, chiP and chiQ, involved in chitin degradation in Pseudoalteromonas sp. strain S91 are described. ChiA, ChiB and ChiC were found to be similar to chitinases belonging to family 18 of the glycosyl hydrolases while ChiP and ChiQ belonged to family 20. Moreover, ChiP and ChiQ exhibited different enzymic activities against fluorescent chitin derivatives, although neither was able to degrade colloidal chitin.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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