Specific detection of Candida albicans and Candida tropicalis by fluorescent in situ hybridization with an 18S rRNA-targeted oligonucleotide probe
Article Abstract:
A non-culture approach for the specific detection of pathogenic fungal species, Candida albicans and C. tropicalis, was investigated. The method used in situ hybridization with fluorescently labelled 020 oligonucleotide probes which binds to the 18S rRNA of C. albicans and C. tropicalis. RNA was extracted from 47 fungal strains and hybridized with the probes at 43 degrees Centigrade. C. albicans and C. tropicalis reacted strongly with fluorescently labelled probe 020. Adherence assay using human umbilical vein endothelial cells showed discrimination between C. albicans and C. krusei, and only C. albicans cells were fluorescent. Germ tubes and hyphae from C. albicans were also fluorescently labelled after in situ hybridization.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Cloning of Candida albicans genes conferring resistance to azole antifungal agents: characterization of CDR2, a new multidrug ABC transporter gene
Article Abstract:
A new ATP-binding cassette (ABC) transporter gene called CDR2 (Candida drug resistance) whose protein confers resistance to azole antifungal agents was identified. The gene was isolated by complementation of fluconazole hypersusceptibility of a Saccharomyces cerevisiae mutant lacking the ABC transporter Pdr5p. The disruption of CDR2 in C.albicans strain CAF4-2 did not render cells more susceptible to azole antifungal agents. CDR2 was overexpressed in isolates which became resistant to these agents.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Acid proteinase secreted by Candida tropicalis: functional analysis of preproregion cleavages in C. tropicalis and Saccharomyces cerevisiae
Article Abstract:
The maturation and processing of the pepsin-like enzyme secreted aspartyl proteinase in strains of Candida tropicalis and Saccharomyces cerevisiae expressing wild-type or mutated forms of the enzyme is investigated. Results indicate that KEX2-dependent proteolytic cleavage is instrumental in coverting the glycosylated 46kDA proenzyme to the enzyme's mature 40kDA form. Moreover, the secretion of the enzyme can take place only upon the removal of the proregion in C. tropicalis and S. cerevisiae.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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