Spectrofluorometric assay for rapid detection of total and fecal coliforms from surface water
Article Abstract:
Determining the presence of fecal coliforms in water on the basis of the fluorescence produced by coliforms' enzymes is easier and faster, if a spectrometer is used instead of UV light. Using the spectrometer the incubation period required before any fluorescence is seen, is 12 hours instead of the 24 hours necessary if UV light is used. The tubes containing the enzymes in lauryl tryptose broth which show emission of more than five fluorescence units contain coliforms, and the tubes which showed emission of more than 10 units contain fecal coliforms.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Detection of frequency resonance energy transfer pair on double-labeled microsphere and Bacillus anthacis spores by flow cytometry
Article Abstract:
Research describes development of an ultrasensitive biosensor using frequency resonance energy transfer and cytometry. Data indicate that the selectivity of the Bacillus anthracis spores is improved by a factor of 10 than those of B. thuringiensis subsp. israelensis and by 100 than those of B. subtilis spores as shown by the fluorescence-labeled avidin molecules on biotin-coated microspheres and bacterial spore antibody interaction.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Observation of soft contact lens disinfection with fluorescent metabolic stains
Article Abstract:
Results reveal that 30 min disinfection of the soft contact lenses with 3% hydrogen peroxide leaves 11 to 13% of cells on lenses.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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