Stereospecific hydroxylation of indan by Escherichia coli containing the cloned toluene dioxygenase genes from Pseudomonas putida F1
Article Abstract:
The Pseudomonas putida F1 todC1C2BA genes code for toluene dioxygenase, which initiates the degradation of toluene, as well as the catalysis of indan oxidation to (-)-(1R)-indanol and trans-1,3-indandiol. The todC1C2BA genes were transferred to Escherichia coli and the indan oxidation reaction catalyzed in this system was compared to that in P. putida. The results showed that E. coli oxidized indan to (-)-(1R)-indanol (83% R), while the indanol product of P. putida was (-)-(1R)-indanol (96% R). This difference was shown to be due to the presence of 1-indanol dehydrogenase in P. putida, which preferentially oxidizes (+)-(1S)-indanol.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Conversion of cis unsaturated fatty acids to trans, a possible mechanism forthe protection of phenol-degrading Pseudomonas putida P8 from substrate toxicity
Article Abstract:
The effects of phenol on the lipid constituents of Pseudomonas putida P8 membranes were studied. Chromatographic and spectrometricexperiments showed that phenol induces cis-to-trans isomerization of fatty acids. One particular fatty acid identified was 9-trans hexadecanoic acid. Thiscis-to-trans isomerization was shown to be independent of lipid biosynthesis, and is catalyzed by a yet unknown cis-trans isomerase. Such cis-trans isomerization may serve to reduce membrane fluidity and permeability, as a protective mechanism against phenolic compounds.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Ratios of carbon isotopes in microbial lipids as indicator of substrate usage
Article Abstract:
A study on the discrimination of the stable carbon isotopes by different microorganisms were made to determine whether ratios of carbon isotopes in microbial lipids can be used as indicators of substrate usage. Eleven strains of well known bacterial and fungal species with four different isotopically define carbon sources were grown and their isotope ratios of fatty acids of different lipid fractions were identified. Results show that the isotope ratio does not depend on the growth stage.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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