Heterogeneity of iron bioavailability on plants assessed with a whole-cell GFP-based bacterial biosensor
Article Abstract:
Heterogeneity of iron bioavailability is discussed based on study in which plants were assessed with a whole-cell green fluorescent protein (GFP)-based bacterial biosensor. Knowledge of variability in iron bioavailability to individuals is required to assess the nature of iron competition in natural habitats. Pseudomonas syringae ferric iron availability was evaluated by measuring the fluorescence intensity of single cells which have a plasmid-borne transcriptional fusion of an iron-regulated promoter from a locus that encodes a membrane receptor for a pyoverdine siderophore with a reporter gene encoding GFP after fluorescence microscopy.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
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Survival of, and induced stress resistance in, carbon-starved Pseudomonas fluorescens cells residing in soil
Article Abstract:
Exponentially growing carbon-starved Pseudomonas fluorescens cells develop resistance to lethal conditions such as ethanol exposure and high temperature when placed in different soils. The carbon-starved P. fluorescens cells placed in Ede loamy sand show a decrease in the inoculant cell and CFU count and an increase in nonculturability, as compared to those placed in Flevo silt loam. The decrease in cell length is the same in both soils. Glucose increases the survival of the bacteria and prevents the decrease in cell size.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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