Synthesis of poly(3-hydroxybutyrate-Co-3-hydroxyvalerate) from methanol and n-amyl alcohol by the methylotrophic bacteria Paracoccus denitrificans and Methylobacterium extorquens
Article Abstract:
Paracoccusdenitrificans and Methylobacterium extorquens were grown together in nitrogen-deficient media supplemented with methanol and n-amyl alcohol, resulting in the synthesis of the copolyester poly(3-hydroxybutyrate co-3-hydroxyvalerate). Analysis of the copolyester content as percentage of dryweight showed that the two strains produced copolyesters composed of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units. P. denitrificans produced copolyesters richer in 3HV. Therefore, this bacterium may be used to synthesize copolyesters with diverse relative proportions of 3HV and 3HB.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Growth-associated production of poly(3-hydroxyvalerate) from n-pentanol by a methylotrophic bacterium, Paracoccus denitrificans
Article Abstract:
The methylotrophic bacterium Paracoccus denitrificans, prepared through the methylmalonyl-CoA, produces a polyester poly(3-hydroxyvalerate) (P(3HV)), when grown on n-pentanol. The increase in the 3HV units during growth leads to the formation of P(3HV). The concentration of n-pentanol affects the production of the polyester. The P(3HV) is dextrorotatory and its tensile strength is lower than the poly(3-hydroxybutyrate) homopolyester.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor
Article Abstract:
Respiration rates of Methylobacterium extorquens AM1 was measured by an optical method to detect oxygen concentrations by using a robust phosphorescence life-time based sensor. Such a method of measuring respiration rates of bacterial cultures could be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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