Thiamin auxotrophy in yeast through altered cofactor dependence of the enzyme acetohydroxyacid synthase

Article Abstract:

Acetohydroxyacid synthase mediates biosynthesis of isoleucine and valine, using thiamin pyrophosphate as a cofactor. Thiamin auxotrophic phenotype of yeast results from an altered dependence for the cofactor due to thi1 mutation, which leads to a substitution of aspartate 176 that is involved in thiamine pyrophosphate binding by the enzyme.

Statistical Data Included, Enzymes, Amino acids, Enzyme structure-activity relationships, Amino acid structure-activity relationships

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The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine

Article Abstract:

Phylogenetic analysis indicate that the THI5 gene family exists exclusively in the yeasts belonging to the Saccharomyces sensu stricto subgroup. Phenotypic analyses of the single, double, triple and quadruple deletion mutants reveal that four genes are functionally redundant with respect to hydroxymethylpyrimidine formation for thiamin biosynthesis. Comparative analysis of mRNA indicates subtle differences in the regulation of the genes.

Goods & services distribution, Channels of Distribution, Influence, Gene mutations, Gene mutation, Genetic aspects, Distribution, Microbiological synthesis, Genetic regulation, Saccharomyces, Heredity, Company distribution practices, Genes

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The DNA and RNA polymerase genes of yeast plasmid pGKL2 are essential loci for plasmid integrity and maintenance

Article Abstract:

The transcriptionally active genes, ORFs, of the cytoplasmic linear killer plasmid pGKL2 of Kluyveromyces lactis are vital for the integrity or regulation of the linear plasmid system. The DNA polymerase gene ORF2 product participates in plasmid replication and indirectly affect the supporting activity for the RNA polymerase gene ORF6 product.

Plasmids, RNA polymerases, DNA polymerases

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