The aflatoxin biosynthesis cluster gene, aflX, encodes an oxidoreductase involved in conversion of versicolorin A to demethylsterigmatocystin
Article Abstract:
Three enzymes encoded by verA, Ver-1 and aflY are required for conversion of versicolorin A (VA) to demethylsterigmatocystin and a study was conducted to show that a fourth enzyme encoded by a previously uncharacterized gene, aflX (ordB) is also required for this conversion. The protein encoded by aflX is predicted to have domains typical of an NADH-dependent oxidoreductase and has 27% amino acid identity to a protein encoded by the aflatoxin cluster gene, aflO (avfA).
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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An aflatoxin biosynthesis cluster gene encodes a novel oxidase required for conversion of versicolorin A to sterigmatocystin
Article Abstract:
The disruption of the aflatoxin biosynthesis cluster gene aflY (hypA) has given Aspergillus parasiticus transformants that accumulated versicolorin A. The gene is found to encode the Baeyer-Villiger oxidase that is necessary for formation of the xanthone ring of the aflatoxin precursor demethylsterigmatocystin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation
Article Abstract:
An aflatoxin cluster gene upstream of the polyketide synthase gene, cypA, which encoded the cytochrome P450 monooxygenase required for formation of G aflatoxins, is discussed. The substrate for cypA oxidation that is required to produce the G aflatoxins is not yet identified.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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