The effect of growth and starvation on the lysis of the ruminal cellulolytic bacterium Fibrobacter succinogenes
Article Abstract:
Cells of the ruminal cellulolytic bacterium Fibrobacter succinogenes in the stationary phase have a ten-fold lower lysis rate than growing cells. This indicates that lysis is not a response to starvation. Autolysis increases the maintenance energy of growing cells. A proteinase that is sensitive to phenylmethylsulfonyl fluoride reduces the autolytic activity of cells in the stationary phase. The lysis rate is seven times higher than the true maintenance rate. However, mathematical calculations suggest that the lysis rate is unaffected by growth rate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Assessment of fecal bacteria with bile acid 7alpha-dehydroxylating activity for the presence of bai-like genes
Article Abstract:
The physiology and genetics of 7alpha-dehydroxylating (7alphaDeOH) intestinal bacteria were analyzed by utilizing deoxyribonucleic acid (DNA) probes. The 7alphaDeOH intestinal bacteria strains exhibited variable cholic acid 7alpha-DeOH activity that can be divided by whole-cell assays into high and low activity groups. Furthermore, Southern blotting also showed that DNA from seven of the 13 strains of 7alphaDeOH hybridized with one bai gene probe while five strains showed no detectable hybridization with any of the six bai gene probes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Identification and characterization of a bile acid 7alpha-dehydroxylation operon in Clostridium sp. strain TO-931, a highly active 7alpha-dehydroxylating strain isolated from human feces
Article Abstract:
The identification of the bacterial enzymes that convert cholic acid to deoxycholic acid will help scientists develop drugs to block this conversion. Deoxycholic acid is toxic and may increase the risk of gallstones and colon cancer. Clostridium sp. strain TO-931 has the highest cholic acid 7alpha-dehydroxylation activity of any known intestinal bacterium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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- Abstracts: Effect of UV radiation on the bacterivory of a heterotrophic nanoflagellate. 714082Quantifying substrate uptake by individual cells of marine bacterioplankton by catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography
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