The glucose transport system of the hyperthermophilic anaerobic bacterium Thermotoga neapolitana

Article Abstract:

In the presence of an external source of energy Thermotoga neapolitana accumulates the nonmetabolizable glucose analog, 2-deoxy-D-glucose against a concentration gradient. This indicates the presence of an active transport system for glucose in the hypertheromophilic anaerobic marine bacterium. 2-deoxy-D-glucose is accumulated in an intracellular free sugar pool that is exchangeable for extracellular D-glucose. The transport depends on the presence of Na+ ion and is inhibited by arsenate and gramicidin D. There is absence of any sugar phosphotransferase activity in T. neapolitana cells.

Analysis, Biological transport, Active, Active biological transport, Anaerobic bacteria

---

Improved methods for cultivation of the extremely thermophilic bacterium Thermotoga neapolitana

Article Abstract:

The suitability of Thermotoga neapolitana for basic molecular biological studies of extreme thermophiles is discussed. Its growth medium components and cultivation conditions were optimized by a formulated defined marine salts medium. Mean plating efficiencies were calculated under aerobic and anaerobic conditions. Its ability to grow on both solid and liquid media with minimal anoxic precautions and its variability despite being a strict anaerobe determined the importance of this evolutionary bacterium.

Methods, Molecular biology

---

Catabolite repression in the hyperthermophilic bacterium Thermotoga neapolitana is independent of cAMP

Article Abstract:

Catabolite repression in the hyperthermophilic bacterium Thermotoga neapolitana is investigated. Results indicate that the bacterium can regulate beta-galactosidase synthesis and exhibit diauxic growth in media containing glucose and lactose. Moreover, it was shown that different sugars affect beta-galactosidase expression in different ways and that cAMP does not play a role in beta-galactosidase regulation.

Gram-negative bacteria, Metabolic regulation, Cyclic adenylic acid, Cyclic adenosine monophosphate

Similar abstracts:

• Abstracts: An extremely thermostable cellulase from the thermophilic eubacterium Rhodothermus marinus. Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats
• Abstracts: Development of a genetic transformation system for an alga-lysing bacterium. Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp strain A28
• Abstracts: Ennoblement of stainless steel by the manganese-depositing bacterium Leptothrix discophora. Enzymatic iron oxidation by Leptothrix discophora: identification of an ironoxidizing protein
• Abstracts: Light-dependent degradation of nitrophenols by the phototrophic bacterium Rhodobacter capsulatus E1F1. Characterization of a nitrophenol reductase from the phototrophic bacterium Rhodobacter capsulatus E1F1
• Abstracts: Nisin dissipates the proton motive force of the obligate anaerobe Clostridium sporogenes PA 3679. Nisin resistance in Listeria monocytogenes ATCC 700302 is a complex phenotype

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.