The recombinant antigen ASPND1r from Aspergillus nidulans is specifically recognized by sera from patients with aspergilloma

Article Abstract:

A 996 bp Aspergillus nidulans cDNA which encodes the ASPND1 immunodominant antigen was cloned and expressed in Escherichia coli as a fusion protein with the enzyme glutathione S-transferase (GST) from Schistoma japonicum. The GST-ASPND1 fusion protein was purified and after cleavage with thrombin, the ASPND1 recombinant antigen (ASPND1r) and the GST protein were separated and immunobloted with several human sera. The sera from 22 out of 25 patients with an aspergilloma recognized the ASPND1r recombinant antigen on immunoblots. Forty-nine normal human sera and 14 sera from patients with other infections were unreactive.

Bacterial antigens, O antigens, Immunodiagnosis, Fungal antigens

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Mutational analysis of AREA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the "streetwise" GATA family of transcription factors

Article Abstract:

Research was conducted to survey and annotate 109 mutant sequences in the areA gene of the ascomycete fungus Aspergillus nidulans (AREA) DBD which include both nonfunctional and functional mutant AREA forms. Corresponding sequence changes were noted in other GATA factor DBDs. Results indicate tolerance to sequence change, mutational changes in a transcription factor can have differing effects on structural gene expression and a mutational change that changes local hydrophobic packing can affect the behavior of mutational changes elsewhere in the DBD.

Fungi, Genetic transcription, Transcription (Genetics), Microbial mutation

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