The topology of the kinetoplast DNA network
Article Abstract:
Experimental analyses of the topology of kinetoplast DNA (kDNA) network that comprises of about 5000 catenated DNA minicircles and about 25 maxicircles reveal that on an average each minicircle associates with three other minicircles. Models of the network structure developed from a graph theory yield values for the frequency of minicircle oligomers resulting from random network breakage. The level of digestion helps determine the fragmentation pattern of the networks. The models predict a honeycomb arrangement and indicate an average valence of 3.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation
Article Abstract:
Chiral knotting by a type II topoisomerase results from the addition of 13S condensin to nicked DNA. Analysis shows that the knots formed from the addition of 13S condensin were almost exclusively trefoils and their typology was highly informative. The creation of three crossing knots suggests that condensin establishes an ordered array of solenoidal supercoils. For efficient knotting to occur, the ATP hydrolysis and the mitotic form of 13S condensin are needed.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila barren protein
Article Abstract:
An immunoaffinity approach utilizing antibodies against XCAP-C and XCAP-E was utilized for the purification and analysis of the chromosome condensation protein complexes. Analysis of the XCAP-C and XCAP-E multiprotein complexes indicated the presence of two major forms of condesins that were homologous to the Xenopus SMC family of proteins. Furthermore, the condesin complexes were characterized as components of the chromosome assembly machinery.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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