Use of bioluminescence for detection of genetically engineered microorganisms released into the environment
Article Abstract:
The use of bioluminescence to monitor the movement of limited field-released strain JS414 of Xanthomonas campestris pv. campestris was described. In this study, the bacteria were added to plant and soil samples by mist and wound inoculation, and by scattering infested debris among plants. The samples were assayed by colony determinations via serial dilution and bioluminescence measurements using a luminometer. The results showed that bioluminiscent X. campestris pv. was detected in both plant and soil samples. The method was sensitive as the plating method but non-labor intensive.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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A new computational method for detection of chimeric 16S rRNA artifacts generated by PCR amplification from mixed bacterial populations
Article Abstract:
A new computational technique for detecting chimeric 16S rRNA artifacts produced during PCR amplification from mixed bacterial populations was developed. The chimeric alignment technique employs the score from dynamic programming alignments. Moreover, the chimeric alignments are shown to the user to aid in sequence classification. The distribution of improvement scores for 500 authentic, nonchimeric sequences and 300 artificial chimeras was utilized to analyze the sensitivity and accuracy of chimeric alignment and CHECK_CHIMERA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Gene copy number polymorphisms in an arbuscular mycorrhizal fungal population
Article Abstract:
Gene copy number polymorphism was studied in a population of the arbuscular mycorrhizal fungus (AMF) Glomus intraradices. The isolates of AMF Glomus intraradices were found to contain genes for adaptation to environmental and pathological conditions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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- Abstracts: Isolation of nitrogen-fixing bacteria containing molybdenum-independent nitrogenases from natural environments
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