Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification
Article Abstract:
Three consensus tRNA primers developed to distinguish between genus and species levels of Pseudomonas solanacearum were tested for their ability to resolve relationships between strains and a closely related species, Pseudomonas syzygii. Analysis of the DNA fingerprint patterns showed that the primers couldresolve three fingerprint groups correlating well with previous classificationsusing restriction fragment length polymorphism analysis. Therefore, the consensus primers may be used in the rapid determination of P. solanacearum subgroups.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains
Article Abstract:
Consensus amino acid sequences of gyrases from different bacteria help construct degenerate PCR primers, which help amplify gyrB genes from different types of bacteria. These amplified fragments help determine the nucleotide sequences of the amplified gyrB DNA from three Pseudomonas putida. The gyrB fragments from P. putida are amplified with the help of a specific set of PCR primers.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Isolation, characterization, and avenacin sensitivity of a diverse collection of cereal-root-colonizing fungi
Article Abstract:
The oat root saponin avenacin A-1 may determine which fungi colonize the roots. In a study of 161 fungal isolates from oat roots, most were avenacin A-1 resistant. Fungi isolated from non-saponin-producting wheat roots contained a mix of avenacin A-1 resistant and avenacin A-1 sensitive types.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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