Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: comparison of induction level and promoter strength

Article Abstract:

Research was conducted to examine the regulated activity of the lactococcal nisA promoter in strains of gram-positive species Streptococcus pyogenes, Streptococcus pneumoniae, Streptococcus agalactiae, Bacillus subtilis and Enterococcus faecalis. Results reveal that the lactococcal nisA promoter is recognized in many different gram-positive species which include pathogenic streptococci. Evidence also demonstrates that it was the most efficient of the regulatable promoters studied in B. subtilis and S. pyogenes.

Bacterial proteins

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Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin

Article Abstract:

The kinetics of induction of the nisin A (nisA) gene in Lactococcus lactis was investigated by comparison with the expression of the gusA gene in L. lactis NZ9800, which contains a deletion in the nisA gene. Transcriptional and translational fusion with a nisA promoter revealed a significant relationship between the amount of nisin in the medium and beta-glucoronidase activity, indicating that enzyme production is linearly dependent on nisA expression.

Analysis, Genetic aspects, Lactobacillus

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Excision of the conjugative transposon Tn916 in Lactococcus lactis

Article Abstract:

Empirical evidence suggests that Lactococcus lactis lacks a host factor necessary for excision and conjugative transposition. This is apparent in the restriction of Lactococcus lactis excision of Tn916 by the concentration of integrase and increase of excision by the addition of more excisionase. Despite the increased excision of Tn916 in Lactococcus lactis, no conjugative transfer can be detected.

DNA, Bacteria, Transposons

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