Utilization of hemin and hemoglobin by Vibrio vulnificus biotype 2
Article Abstract:
The eel pathogen Vibrio vulnificus biotype 2 uses hemoglobin (Hb) and hemin (Hm) to obtain iron. The mechanism involves direct interaction with the heme moiety and outer membrane proteins (OMP). The binding of bacterial cells with Hm and Hb is independent of the iron levels in the medium and the presence of polysaccharides on the bacterial surface. Western blotting experiment of OMPs reveals two proteins which bind with Hm and Hb. This protein expression is independent of the iron levels. V. vulnificus biotype 2 produces extracellular proteases that act against Hb.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Restriction fragment length polymorphisms and sequence analysis: an approach for genotyping infectious pancreatic necrosis virus reference strains and other aquabirnaviruses isolated from Northwestern Spain
Article Abstract:
Infectious pancreatic necrosis virus (IPNV) is an unenveloped icosaedrical virus found in fishes of Spain. Restriction fragment length polymorphism (RFLP) analysis yielded seven geno groups and further analyses suggested that the two-step restriction analysis assay is the best method for typing new isolates of aquabirnaviruses aquabirnaviruses.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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