Virus passage through track-etch membranes modified by salinity and a nonionic surfactant
Article Abstract:
Factors other than stereohindrance obstruct the passage of viruses through track-etch membranes. This is evident in an experiment which determined virus transmission as a function of salinity and with or without a nonionic surfactant. It was shown that increasing the salinity reduced phiX174 and PRD1 passage through track-etch polycarbonate membranes as well as PRD1 passage through polyester membranes. Virus passage was reduced by high levels of salinity through reduction of electrostatic repulsion, which prevented adsorption in the process.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Elution of viruses by ionic and nonionic surfactants
Article Abstract:
A study evaluating the bacteriophages phi-X174 and PRD1 shows that the ionic surfactant sodium dodecyl sulfate (SDF) and the nonionic surfactant Triton X-100 elutes these two viruses. Triton X-100 and SDF also absorb the viruses to the nitrocellulose and the positively charged membranes. Triton X-100 completely elutes the viruses to positively charged membranes, whereas SDF does the same to nitrocellulose membranes. The elution process involves the reduction of surface tension by the surfactants, thereby facilitating the process.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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A simple method to test condoms for penetration by viruses
Article Abstract:
A simple method to evaluate the effectiveness of condoms as virus barriers was developed. The procudure involves the use of phi-X174 as the challenge virus which is presented under physiologically relevant pressures. Virus penetration is determined by analyzing aliquots taken from a collecting vessel. Tests on the newly developed procedure showed that penetration of 1 microliter or less of challenge suspension could be detected.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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