WTI interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes
Article Abstract:
The WT1 gene, which is required for normal kidney development, in isoforms, has functional significance. It appears that WT1 has a part to play in pre-mRNA splicing. Genetic irregularities are associated with Frasier syndrome, Denys Drash syndrome (DDS), and Wilms' tumor.WT1 is usually thought of as a transcription factor, but it interacts with a required splicing factor called U2AF65 and it associates with the splicing mechanisms. WT1 is spliced in alternative ways. Isoforms that have three amino acids among them have more vigorous interaction with U2AF65 and better colocalization with splicing factors in vivo.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
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Influence of PAX6 gene dosage on development: overexpression causes severe eye abnormalities
Article Abstract:
Transgenic mice were generated using a 420 kb PAX6 yeast artificial chromosome for an in-depth study of gene dosage effects. Observations show that the transgene rescues the mutant phenotype when crossed onto the 'Small eye' background. Mice carrying multiple copies on a wild-type background were found to have specific developmental abnormalities of the eye. Other tissues expressing the gene were unaffected. Reduced and increased levels of transcriptional regulators were found to cause developmental defects.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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Subnuclear localization of WT1 in splicing or transcription factor domains is regulated by alternative splicing
Article Abstract:
Tumor predisposition gene (WT1) plays an important role in transcriptional and posttranscriptional RNA processing. WT1 and the splicing factor colocalize in speckles and coiled bodies. An immunoprecipitation shows that WT1 isoforms interact directly with one or more components of splicing machinery and COS studies suggest that WT1 associates mainly with DNA in transcription factor domains.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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