When coupled to natural transformation in Acinetobacter sp. strain ADP1, PCR mutagenesis is made less random by mismatch repair
Article Abstract:
Transformation-facilitated random PCR mutagenesis is successfully applied to proteins involved in the catabolism and regulation of phytochemicals in the nutritionally versatile Acinetobacter sp. strain. Random PCR mutagenesis is a powerful tool for structure-function analysis of targeted proteins, especially when coupled with DNA integration through natural transformation followed by selection for loss of function.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Toxicity caused by hydroxycinnamoyl-coezyme a thioester accumulation in mutants of acinetobacter sp. strain ADP1
Article Abstract:
A key step in the hca-encoded pathway is activation of the natural substrates caffeate, p-coumarate, and ferulate by an acyl:coenzyme A ligase encoded by hcaC. An hcaC mutation from one isolate is characterized and is found to result in the substitution of an amino acid that is conserved in a functionally characterized homolog of HcaC.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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