Human C5aR knock-in mice facilitate the production and assessment of anti-inflammatory monoclonal antibodies

Article Abstract:

Neutrophils from human C5aR knock-in mice, in which the mouse C5aR coding region is replaced with that of human C5aR is used to immunize wild-type mice and to generate high-affinity antagonist monoclonal antibodies (mAbs) to human C5aR. Results reveal that of ~40 mAbs generated to C5aR, all potent inhibitors recognized a small region of the second extracellular loop that seems to be critical for regulation of receptor activity.

Inflammation, Inflammation mediators, Structure

---

Rapid generation of specific antibodies by enhanced homologous recombination

Article Abstract:

The rapid generation of specific monoclonal antibodies using the chicken DT40 B-cell line undergoing gene conversion is reported. It is discovered that the gene conversion frequency at the immunoglobulin locus is increased by treating DT40 cells with a histone deacetylase inhibitor, trichostatin A, thereby generating diversity at the immunoglobulin locus in the majority of treated cells.

B cells

---

Simultaneous targeting of multiple disease mediators by a dual-variable-domain immunoglobulin

Article Abstract:

A generally applicable model of a dual-specific, tetravalent immunoglobulin G (IgG)-like molecule (dual-variable-domain Ig (DVD-Ig)) is developed, which is engineered from two monoclonal antibodies while preserving activities of parental antibodies. The studies of DVD-Ig protein in an animal disease model has shown that it can be used as therapeutics in human diseases.

Viral antibodies, Antibodies

Similar abstracts:

• Abstracts: Mimetic entry and bandwagon effect: the rise and decline of international equity venture in China. Organizational slack and firm performances during economic transitions: two studies from an emerging economy
• Abstracts: Compliance to owner's manual warnings: influence of familiarity and the placement of a supplemental directive
• Abstracts: Simulation based stress tests of banks' regulatory capital adequacy. Backtesting for risk - based regulatory capital
• Abstracts: How rating agencies achieve rating stability. The importance and subtlety of credit rating migration. Rating the rating agencies: anticipating currency crisis or debt crisis?
• Abstracts: Enhancing service personnel effectiveness through the use of behavioral repertoires. A marketing-relevant framework for understanding service worker productivity

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.